1

蛋白質體分析

Proteomic analysis

1-1

丙酮沉澱 (若蛋白回溶體積過大或有干擾物建議進行此步驟)

Acetone precipitation (recommended if the protein resuspension volume is too large or contains interfering substances)

500元/樣品

NTD 500/sample

750元/樣品

NTD 750/sample

1-2

蛋白質濃度測定 (Bradford/BCA assay)

Protein concentration measurement (Bradford/BCA assay)

800元/批

NTD 800/batch

1200元/批

NTD 1200/batch

1-3

蛋白質消化 (Trypsin digestion, 含上機前ZipTip去鹽)

Protein digestion (Trypsin digestion, including ZipTip desalting prior to instrument analysis)

800元/樣品

NTD 800/sample

1200元/樣品

NTD 1200/sample

1-4

iRT添加 (DIA method專用)

iRT spike-in (for DIA method only)

600元/樣品

NTD 600/sample

900元/樣品

NTD 900/sample

1-5

蛋白體上機: 一般蛋白質鑑定 (使用Orbitrap Eclipse 45分鐘, 提供Excel表格)

Proteomic analysis on instrument: General protein identification (45 minutes using Orbitrap Eclipse; Excel results provided)

2200元/樣品

NTD 2200/sample

3300元/樣品

NTD 3300/sample

1-6

蛋白體上機: Label-free非標記定量法 (使用Orbitrap Eclipse 68分鐘 DDA method, 提供原始質譜數據)

Proteomic analysis on instrument: Label-free non-labeled quantitative method (68 minutes using Orbitrap Eclipse, DDA method; raw mass spectrometry data provided)

2500元/樣品

NTD 2500/sample

3750元/樣品

NTD 3750/sample

1-7

蛋白體上機: Label-free非標記定量法 (使用Orbitrap Eclipse 68分鐘 DIA method, 提供原始質譜數據)

Proteomic analysis on instrument: Label-free non-labeled quantitative method (68 minutes using Orbitrap Eclipse, DIA method; raw mass spectrometry data provided)

2800元/樣品

NTD 2800/sample

4200元/樣品

NTD 4200/sample

1-8

基本ID定量分析 (Proteome Discoverer or Spectronaut 提供Excel定量表格)

Basic ID and quantification analysis (using Proteome Discoverer or Spectronaut; Excel quantification results provided)

2500元/次

NTD 2500/session

3750元/次

NTD 3750/session

1-9

進階差異蛋白體分析 (含差異表達蛋白質清單, Volcano plot & GO pathway 分析)

Advanced differential proteomic analysis (includes list of differentially expressed proteins, Volcano plot, and GO pathway analysis)

5000元/次

NTD 5000/session

7500元/次

NTD 7500/session

1-10

大量DDA定量樣品分析: 30個以上 (含1-3, 1-6, 1-8, 1-9)

Large-scale DDA quantitative sample analysis:  Over 30 samples (including combinations such as 1-3, 1-6, 1-8, 1-9)

4000元/樣品

NTD 4000/sample

6000元/樣品

NTD 6000/sample

1-11

大量DIA定量樣品分析: 30個以上 (含1-3, 1-7, 1-8, 1-9)

Large-scale DIA quantitative sample analysis: Over 30 samples (including combinations such as 1-3, 1-7, 1-8, 1-9)

5000元/樣品

NTD 5000/sample

7500元/樣品

NTD 7500/sample

2

非標靶代謝體

Untargeted metabolome

2-1

代謝體萃取 (目前僅提供血液, 尿液, 植物組織之服務, 若有其他樣品種類之萃取需求須先與管理者討論)

Metabolite extraction (currently only available for blood, urine, and plant tissue samples. For extraction of other sample types, please consult with the Platform manager in advance)

600元/樣品

NTD 600/sample

900元/樣品

NTD 900/sample

2-2

代謝體質譜分析 (提供質譜原始數據)

Metabolomics mass spectrometry analysis (raw mass spectrometry data provided)

1600元/樣品

NTD 1600/sample

2400元/樣品

NTD 2400/sample

2-3

基本ID定量分析 (MS-DIAL or Compound Discoverer提供Excel定量表格)

Basic identification and quantification analysis (quantification results provided in Excel format using MS-DIAL or Compound Discoverer)

2000元/樣品

NTD 2000/sample

3000元/樣品

NTD 3000/sample

2-4

進階差異代謝體分析 (含差異表達代謝物清單, Volcano plot & KEGG pathway 分析)

Advanced differential metabolomics analysis (includes a list of differentially expressed metabolites, Volcano plot, and KEGG pathway analysis)

5000元/次

NTD 5000/session

7500元/次

NTD 7500/session

2-5

大量樣品分析: 50個以上 (含2-1~2-4)

Large-scale sample analysis: Over 50 samples (including combinations such as 2-1 to 2-4)

4000元/樣品

NTD 4000/sample

6000元/樣品

NTD 6000/sample

3

標靶代謝體

Targeted metabolomics

3-1

特定代謝物樣品萃取 (目前僅提供植物荷爾蒙之萃取, 包含穩定同位素標準品添加, 若有其他樣品種類之萃取需求須先與管理者討論)

Extraction of specific metabolite samples (currently only plant hormone extraction is available, including addition of stable isotope-labeled standards. For other sample types, please consult with Platform manager in advance)

1000元/樣品

NTD 1000/sample

1500元/樣品

NTD 1500/sample

3-2

特定代謝物質譜分析 (提供原始質譜數據及Excel定量表格)

Mass spectrometry analysis of specific metabolites (raw mass spectrometry data and Excel quantification results provided)

1000元/樣品

NTD 1000/sample

1500元/樣品

NTD 1500/sample

3-3

大量特定代謝物質譜分析: 30個樣品以上 (上機+基本ID定量分析)

Large-scale specific metabolite mass spectrometry analysis: Over 30 samples (including instrument run and basic ID/quantification analysis)

1600元/樣品

NTD 1600/sample

2400元/樣品

NTD 2400/sample

3-4

新化合物質譜方法條件建立 (需先與管理者討論文獻方法，並由使用者提供管柱及標準品)

New compound mass spectrometry method development (prior discussion with the manager about literature-based methods is required. Users must provide columns and reagents)

20000元/化合物

NTD 20,000/compound

30000元/化合物

NTD 30000/compound

3-5

新化合物質譜分析 (需提供標準品)

New compound mass spectrometry analysis (reagents must be provided by the user)

1800元/樣品

NTD 1800/sample

2700元/樣品

NTD 2700/sample

21. Targeted metabolomics 標靶代謝體服務項目定量分析一覽表，請詳參辦法附件。

List of quantitative analysis services for Targeted metabolomics, refer to the appendix of these guidelines for the complete list.

4

其他設備使用及諮詢

Other Equipment Use and Consultation

4-1

離心濃縮機使用(自行操作)

Use of centrifugal concentrator (self-operation)

200元/次

NTD 200/session

300元/次

NTD 300/session

4-2

特殊實驗設計諮詢 (樣品種類非上述所列項目, 皆為特殊實驗設計)

Special experimental design consultation (any sample types not listed above are considered special designs)

請洽平台管理人

Please contact Platform manager

請洽平台管理人

Please contact Platform manager